Table S1

ID	Reaction name	Kinetic parameters			References
a1	PKC_bind_Ca	k_f = 0.25 sec^-1mM^-2	k_b = 1 sec^-1	K_d = 2 mM	1
a2	PKC-Ca_memb	k_f = 1 sec^-1	k_b = 0.1 sec^-1	K_eq = 10	1
a3	PKC_bind_AA	k_f = 0.2 sec^-1mM^-1	k_b = 10 sec^-1	K_d = 50 mM	2
a4	PKC-AA_bind_Ca	k_f = 0.25 sec^-1mM^-2	k_b = 1 sec^-1	K_d = 2 mM	1
a5	PKC-Ca_bind_AA	k_f = 0.2 sec^-1mM^-1	k_b = 10 sec^-1	K_d = 50 mM	2
b1	PKC_phos_Raf	K_m = 11.5 mM	k_cat = 0.0335 sec^-1	k_-1/k_cat = 4	3, 4
b2	PP2A_deph_Raf-P	K_m = 15.7 mM	k_cat = 6 sec^-1	k_-1/k_cat = 4	5
b3	Raf_act_MEK	K_m = 0.398 mM	k_cat = 0.105 sec^-1	k_-1/k_cat = 4	6
b4	Raf_act_MEK-P	K_m = 0.398 mM	k_cat = 0.105 sec^-1	k_-1/k_cat = 4	6
b5	PP2A_deph_MEK-PP	K_m = 15.7 mM	k_cat = 6 sec^-1	k_-1/k_cat = 4	5, 7, 8
b6	PP2A_deph_MEK-P	K_m = 15.7 mM	k_cat = 6 sec^-1	k_-1/k_cat = 4	5, 7, 8
b7	MEK_act_MAPK	K_m = 0.0463 mM	k_cat = 0.15 sec^-1	k_-1/k_cat = 4	9, 10
b8	MEK_act_MAPK-P	K_m = 0.0463 mM	k_cat = 0.15 sec^-1	k_-1/k_cat = 4	9, 10
b9	MKP_deph_MAPK-PP	K_m = 0.16667 mM	k_cat = 1 sec^-1	k_-1/k_cat = 4	11, 12
b10	MKP_deph_MEPK-P	K_m = 0.16667 mM	k_cat = 1 sec^-1	k_-1/k_cat = 4	11, 12
c1	MAPK_phos_PLA2	K_m = 25.6 mM	k_cat = 20 sec^-1	k_-1/k_cat = 4	13, 14
c2	PLA2-P_deg	k_f = 0.17 sec^-1	k_b = 0 sec^-1		15
c3	PLA2_bind_Ca	k_f = 0.01 sec^-1	k_b = 0.1 sec^-1	K_d = 10 mM	16
c4	PLA2-PIP2_bind_Ca	k_f = 0.01 sec^-1	k_b = 0.1 sec^-1	K_d = 10 mM	16
c5	PLA2_bind_PIP2	k_f = 0.0012 sec^-1	k_b = 0.48 sec^-1	K_d = 400 mM	4
c6	PLA2-Ca_bind_PIP2	k_f = 0.0012 sec^-1	k_b = 0.48 sec^-1	K_d = 400 mM	4
c7	PLA2-Ca_prd_AA	K_m = 20 mM	k_cat = 54 sec^-1	k_-1/k_cat = 4	17
c8	PLA2-PIP2_prd_AA	K_m = 20 mM	k_cat = 11.04 sec^-1	k_-1/k_cat = 4	17
c9	PLA2-PIP2-Ca_prd_AA	K_m = 20 mM	k_cat = 36 sec^-1	k_-1/k_cat = 4	17
c10	PLA2-P_prd_AA	K_m = 20 mM	k_cat = 120 sec^-1	k_-1/k_cat = 4	17
c11	AA_deg	k_f = 0.4 sec^-1	k_b = 0 sec^-1		15
d1	PKC_phos_AMPAR	K_m = 3.5 mM	k_cat = 0.05 sec^-1	k_-1/k_cat = 4	4
d2	PP2A_deph_AMPAR-P	K_m = 15.7 mM	k_cat = 6 sec^-1	k_-1/k_cat = 4	5
k_f and k_b are defined by the formula S8. k₁, k_-1, and k_cat are defined by the formula S9. K_d: dissociation constant (K_d = k_b/k_f) K_eq: equilibrium constant (K_eq = k_b/k_f) K_m: Michaelis constant (K_m = (k_-1 + k_cat)/k₁ )

Table S2

Molecular name	Full name	Concentration	References
PKC	Protein kinase C	1 mM	18
PP2A	Protein phosphatase 2A	0.045 mM	19
Raf	Raf	0.5 mM	20
MEK	MAPK or ERK kinase	0.5 mM	21, 22
MAPK	Mitogen-activated protein kinase	1 mM	22, 23
MKP	MAPK phoaphatase	0.0032 mM	24
PLA2	Phospholipase A2	0.4 mM	16
PIP2	Phosphatidylinositol bisphosphate	10 mM	4
APC	Aracholonylphosphatidylcholine	30 mM	25
AMPAR	AMPA receptor	0.5 mM	26

References for Table S1 and S2

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11. Charles, C. H., Abler, A. S., and Lau, L. F. (1992). cDNA sequence of a growth factor-inducible immediate early gene and characterization of its encoded protein. Oncogene 7, 187-190.

12. Charles, C. H., Sun, H., Lau, L. F., and Tonks, N. K. (1993). The growth factor-inducible immediate-early gene 3CH134 encodes a protein-tyrosine-phosphatase. Proc Natl Acad Sci U S A 90, 5292-5296.

13. Sanghera, J. S., Paddon, H. B., Bader, S. A., and Pelech, S. L. (1990). Purification and characterization of a maturation-activated myelin basic protein kinase from sea star oocytes. J Biol Chem 265, 52-57.

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18. Marquez, C., Martinez, C., Kroemer, G., and Bosca, L. (1992). Protein kinase C isoenzymes display differential affinity for phorbol esters. Analysis of phorbol ester receptors in B cell differentiation. J Immunol 149, 2560-2568

19. Mumby, M. C., Green, D. D., and Russell, K., L. (1985). Structural characterization of cardiac protein phosphatase with a monoclonal antibody. Evidence that the Mr = 38,000 phosphatase is the catalytic subunit of the native enzyme(s). J Biol Chem 260, 13763-13770

20. Storm, S. M., Cleveland, J. L., and Rapp, U. R., (1990). Expression of raf family proto-oncogenes in normal mouse tissues. Oncogene 5, 345-351.

21. Seger, R., Ahn, N. G., Posada, J., Munar, E. S., Jensen, A. M., Cooper, J. A., Cobb, M. H., and Krebs, E. G. (1992). Purification and characterization of mitogen-activated protein kinase activator(s) from epidermal growth factor-stimulated A431 cells. J Biol Chem 267, 14373-14381.

22. Huang, C. Y., and Ferrell, J. E., Jr. (1996). Ultrasensitivity in the mitogen-activated protein kinase cascade. Proc Natl Acad Sci U S A 93, 10078-10083.

23. Sanghera, J. S., Paddon, H. B., Bader, S. A., and Pelech, S. L. (1990). Purification and characterization of a maturation-activated myelin basic protein kinase from sea star oocytes. J Biol Chem 265, 52-57.

24. Sun, H., Charles, C. H., Lau, L. F., and Tonks, N. K. (1993). MKP-1 (3CH134), an immediate early gene product, is a dual specificity phosphatase that dephosphorylates MAP kinase in vivo. Cell 75, 487-493.

25. Wijkander, J., and Sundler, R., (1991). An 100-kDa arachidonate-mobilizing phospholipase A2 in mouse spleen and the macrophage cell line J774. Purification, substrate interaction and phosphorylation by protein kinase C. Eur J Biochem 202, 873-880.

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